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KMID : 1005220120270040259
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Journal of Embryo Transfer
2012 Volume.27 No. 4 p.259 ~ p.264
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Effects of Osmolality Step during Vitrification on Survival of Vitrified IVP Embryos in Korean Native Cattle (Hanwoo)
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Yoo Han-Jun
Choi Hye-Won
Cheong Ki-Soo
Kim Ji-Tae
Lee Chang-Woo
Park Choon-Keun
Park Joung-Jun
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Abstract
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Solution of glycerol, ethylene glycol, sucrose, dextrose (GESD) and cryotop methods were carried out to investigate the survivability on vitrification of embryos. Embryos cultured in vitro were vitrified by GESD of 10 or 8 step and cryotop methods of 6 step, from cryopreservation step to frozen-thawed and culture step. Survival rate and ICM, TE cells of embryos were investigated after frozen-thawed 24 h. As a results, cryotop method was significantly (p<0.05) higher (85.76 ± 5.3 vs. 66.71 ± 2.4, 44.80 ± 2.1%) than GESD 10 or 8 step methods on survivability. Also, In ICM cell number, cryotop method was significantly (p<0.05) higher to 45.67 ± 4.7 cells than GESD 8 step method. TE cell number was significantly (p<0.05) highest to 111.00 ± 11.0 cells in cryotop method. On the other hand, survival rate, TE and total cell number were all the significantly (p<0.05) high, except ICM in GESD 10 step method between GESD 10 step method and GESD 8 step method. In conclusion cryotop method was to be most effective, but it is considered necessary to study vitrification method for step-by-step freezing and thawing process.
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KEYWORD
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vitrification , GESD , cryotop , osmolality , hanwoo
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